# UW-Madison Biotechnology Center — Knowledge Base This is a curated knowledge base of public information from biotech.wisc.edu, assembled for the **Self-Service KB Assistant** lesson (Lesson 23). It is a snapshot — pages may have been updated since this was scraped on 2026-04-10. For authoritative information, see the linked source URLs. This file is the *only* source the KB assistant should use to answer questions. If a question cannot be answered from the content below, the assistant must say "I don't know" and suggest contacting the relevant facility directly. --- ## Source: https://biotech.wisc.edu/uwbc-services/ ### UW Biotechnology Center Services Overview **Address:** 425 Henry Mall, Madison, WI 53706 **General feedback:** feedback@biotech.wisc.edu **Website issues:** webmaster@biotech.wisc.edu **Administrative IDs:** - UW Fee-For-Service TIN: 39-1805963 - SAM Unique Entity ID: HVEKL7MENUK1 The UW Biotechnology Center operates eight core service centers covering bioinformatics, sequencing, gene expression, genome editing, animal models, mass spectrometry, isotope analysis, and shared wet lab space. All services are intended for research purposes only — none are certified for diagnostic or clinical use. **The eight cores:** - Bioinformatics Resources Core (BRC) - Gene Expression Center (GEC) - DNA Sequencing Facility (NGS / Next Gen Sequencing) - Mass Spectrometry Core - Advanced Genome Editing (AGE) - Animal Models (GEAM) - Isotope Ratio Mass Spectrometry Lab (IRMS) - BioGarage (shared wet lab) A "My Data" portal at biotech.wisc.edu/data-transfer/ handles sample and project data management across the cores. --- ## Source: https://biotech.wisc.edu/contact/ ### Contact Information **Mailing address:** University of Wisconsin Biotechnology Center, 425 Henry Mall, Madison, WI 53706 **Phone:** 608-262-8606 **General feedback:** feedback@biotech.wisc.edu **Website issues:** webmaster@biotech.wisc.edu **Related programs (separate from the cores):** - Biotechnology Training Program: www.btp.wisc.edu - MS in Biotechnology Program: www.ms-biotech.wisc.edu The webmaster email is for website content issues only. Graduate program inquiries and employment inquiries should not be directed there — see the program-specific sites above. The contact page does not list hours, parking, directions, or per-facility leads. For facility-specific contact, see the individual core entries below. --- ## Source: https://biotech.wisc.edu/dna-seq/ ### DNA Sequencing Facility (Next Gen Sequencing) The Next Gen DNA Sequencing Core is a state-of-the-art lab using Illumina, PacBio, and Oxford Nanopore platforms for genomic research. The facility emphasizes collaboration on new projects. **Director:** Joshua R. Hyman, Ph.D. **Location:** Room 1320, Biotechnology Center, 425 Henry Mall, Madison, WI 53706 **Email:** nextgen-seq@biotech.wisc.edu **RRID:** SCR_017759 (cite this in publications) **Sequencing platforms:** - Illumina NovaSeq X Plus - PacBio Sequel II - Oxford Nanopore PromethION **Services:** - Plasmid sequencing and assembly - CRISPR-Cas9 editing analysis - Amplicon sequencing (16S/18S/ITS, custom amplicon, index PCR) - DNA extraction (multiple methods) - Genotyping by sequencing (GBS) and other genotyping - PacBio long-read sequencing - Oxford Nanopore Technologies sequencing - Whole genome sequencing - Exome capture - ChIP-Seq - Quality control services **Operational notes:** The facility observes major holidays (January 1, July 4, Thanksgiving, Christmas) and federal holidays. Limited staffing operates December 24–31, which may impact turnaround times. **Disclaimer:** All data and reagents are intended for research purposes only. Not certified for diagnostic or clinical use. Clinical genomics services are available through the UW Collaborative Genomics Core (a separate facility). --- ## Source: https://biotech.wisc.edu/gec/ ### Gene Expression Center (GEC) The Gene Expression Center delivers cost-effective transcriptomic (bulk, single-cell, spatial) and large-scale genotyping/methylation services to UW researchers and external institutions. **Director:** Sandra Splinter BonDurant, MS **Address:** 2120 Biotechnology Center, 425 Henry Mall, Madison, WI 53706 **Email:** gecinfo@biotech.wisc.edu **RRID:** SCR_017757 **RNA Sequencing & Library Prep:** - Bulk RNA-seq library prep using NEB, Illumina, and Takara short-read kits - PacBio Iso-Seq long-read library prep - Oxford Nanopore long-read library prep **Single-Cell Analysis:** - 10X Genomics Single Cell RNA-Seq - 10X Genomics Single Cell ATAC-Seq - Parse Biosciences Evercode platform - Guidance available via "Guide to Getting Started with 10X Genomics Single Cell RNA-Seq" **Spatial Transcriptomics:** - 10X Genomics Xenium (in situ spatial profiling, subcellular resolution) - 10X Genomics Visium - NanoString GeoMx (in collaboration with the UW Madison TRIP lab) **RNA Quality Control:** Standalone RNA extraction and QC available. **Genotyping & Methylation:** Array-based genotyping, GWAS, and methylation assays via Illumina iScan systems. **Important data policy:** Deliverable data is deleted from GEC systems 60 days after it is made available for download. Plan to retrieve your data promptly. **Acknowledgment language for publications:** "The author(s) utilized the University of Wisconsin–Madison Biotechnology Center Gene Expression Center (RRID:SCR_017757) for [specific service]." Notify gecinfo@biotech.wisc.edu when you publish so the work can be listed on the GEC website. **Disclaimer:** Services are for research purposes only. Not certified for diagnostic or clinical use. --- ## Source: https://biotech.wisc.edu/age/ ### Advanced Genome Editing (AGE) Lab The AGE Lab provides comprehensive genome editing services across cells, organoids, and animal models. **Director:** C. Dustin Rubinstein, Ph.D. **Location:** Room 3122, Biotechnology Center, 425 Henry Mall, Madison, WI 53706 **Email:** genomeediting@biotech.wisc.edu **Services:** - CRISPR-edited mouse, rat, and porcine model generation (in collaboration with the UWBC Animal Models Core) - Genome-edited clonal cell lines and organoids (uses automation and QA instrumentation) - Genome-wide pooled CRISPR screening - Customizable genome editing applications, including therapeutic program development - Whole genome sequencing and long-read sequencing for model characterization - Bioinformatic analysis using CRISPResso2 and CRISPRme **Positioning:** AGE describes its CRISPR animal model service as "faster, less costly, and with unprecedented genomic assurance stringency" compared to traditional approaches. Models are characterized in-house to expedite research timelines. **Events:** AGE hosts the "Organoids in Action" symposium scheduled for April 14, 2026, at the Biotechnology Center. **Note on overlap with GEAM:** AGE handles the CRISPR design and editing; the Animal Models Core (GEAM) handles the embryo manipulation and animal husbandry. CRISPR animal projects typically involve both groups. --- ## Source: https://biotech.wisc.edu/animalmodels/ ### Animal Models Core (GEAM) Founded in 1988 (formerly the Transgenic Animal Facility). Provides researchers with CRISPR/Cas9-based and traditional transgenic animal services. Nationally recognized for rat embryo manipulation expertise. **Director:** Kathy Krentz **Location:** Biotechnology Center, 425 Henry Mall, Madison, WI 53706 - Office: Room 3124 - Laboratory spaces: Rooms 3210, 3220 **Email:** animalmodels@biotech.wisc.edu **RRID:** SCR_024797 **Services:** - Mouse, rat, or swine model generation using CRISPR/Cas9 - Embryo cryopreservation (mice and rats) - Sperm cryopreservation (mice and rats) - Rederivation of rodent models from outside institutions - International acquisition of rodent models from established repositories - Traditional transgenic and ES cell-targeted model generation - Reproductive support: breeding guidance, ovarian transplants, sperm evaluation, fertility testing **Eligibility:** Accepts projects from UW-Madison departments, University of Wisconsin System institutions, U.S. academic organizations, and commercial clients. **Submission forms:** - **Animal Models Submission Form** — for non-CRISPR projects - **GEAM Submission Form** — for CRISPR-based projects **Note:** CRISPR projects typically involve coordination with the Advanced Genome Editing (AGE) Lab. --- ## Source: https://biotech.wisc.edu/massspec/ ### Mass Spectrometry Core Facility The Mass Spec Core analyzes biomolecules including proteins, peptides, oligonucleotides, oligosaccharides, and small organic and inorganic molecules. Beyond molecular weight, the facility performs peptide sequencing, post-translational modification mapping, and proteolytic digestion for peptide mapping, sequencing, and quantification. **Director:** Greg Barrett-Wilt, Ph.D. **Location:** Room 2250, Biotechnology Center, 425 Henry Mall, Madison, WI 53706 **Email:** mass_spec@biotech.wisc.edu **Services:** - Lipidomics analysis (pilot grants available) - Electrospray direct injection - Electrospray LC/MS and LC/MS/MS - Small molecule quantitation - In-gel digestion and protein identification - Solution digestion and protein identification - Targeted quantitative proteomics - Untargeted quantitative proteomics **Instrumentation:** Electrospray ionization (ESI) with HPLC. Supports both reversed-phase (RP) and hydrophilic-interaction (HILIC) chromatography for complex mixture characterization. LC/MS/MS is used for quantitation of known compounds and rapid screening of mixtures. **Eligibility:** Accepts samples from all schools, colleges, and departments on the UW–Madison campus, across the University of Wisconsin System, academic institutions nationwide, and commercial/industry clients worldwide. --- ## Source: https://biotech.wisc.edu/brc/ ### Bioinformatics Resources Core (BRC) The BRC is a cost-recovery facility helping researchers with experimental design, standardized analysis pipelines, and data interpretation. The model is "BRC handles the processing so you can focus on the interpretation." **Address:** 425 Henry Mall, Madison, WI 53706 **Email:** brc@biotech.wisc.edu **RRID:** SCR_017799 **Data analysis services (standardized pipelines):** - Whole genome sequencing analysis - Exome analysis - Targeted variant analysis - RNA-seq analysis - Footprinting studies (ChIP, ATAC, Cut&Run) - Genotype-by-sequencing analysis - 16S/18S/ITS microbial analysis - Genome assembly - AlphaFold protein structure prediction - Custom data wrangling **Consultation rates:** - Internal users: $175 per hour - External users: $291.17 per hour - Direct grant support also available via a percent-effort model **Server & computational access:** - Computer laboratory - Server rental - Access to specialized software and genome databases - Server schedule maintained - Account requests via formal application process **Workshops:** The BRC runs training workshops for bioinformatic techniques. **Acknowledgment:** Cite RRID:SCR_017799 in publications and presentations using BRC services. --- ## Source: https://biotech.wisc.edu/irms/ ### Isotope Ratio Mass Spectrometry Lab (IRMS) — also known as the Schoeller Lab The IRMS Laboratory is a premier core facility specializing in precise measurements of stable isotopes: hydrogen (²H/¹H), carbon (¹³C/¹²C), nitrogen (¹⁵N/¹⁴N), and oxygen (¹⁸O/¹⁶O). Established as a core facility in 2013, the lab traces its foundation to Professor Emeritus Dale Schoeller, who pioneered the doubly labeled water (DLW) method in humans for measuring total energy expenditure (TEE) — a technique still widely used worldwide. **Director:** Dr. Michele N. Ravelli (PhD, MS, RDN) **Senior Research Specialist:** Tim Shriver (25+ years of experience in exercise physiology and IRMS operations) **Location:** Room 2210 (2nd Floor), Biotechnology Center, 425 Henry Mall, Madison, WI 53706 **Services:** - High-precision natural abundance and low-enrichment stable isotope analysis - Nutrition, metabolism, obesity science, and energy expenditure measurements - Doubly labeled water (DLW) method for total energy expenditure (TEE) — gold standard - Ecology, hydrology, nutrient cycling, and food web research - Food and agricultural science investigations (provenance and authenticity) - Environmental studies **Eligibility:** Serves UW-Madison investigators, external academics, industry partners, and collaborators worldwide. **Note:** The public IRMS page does not list a direct facility email or phone. For inquiries, contact through the main UW Biotechnology Center channels (feedback@biotech.wisc.edu, 608-262-8606) and ask to be directed to the IRMS / Schoeller Lab. --- ## Source: https://biotech.wisc.edu/biogarage ### BioGarage — Shared Wet Lab Space The BioGarage is a unit of shared wet lab space within the UW Biotechnology Center that provides affordable access to laboratory space and walk-up instrumentation. Located inside the UWBC building, it serves researchers from both the university and external organizations. **Manager:** Molly Nellen (BioGarage Manager) **Location:** Room 2302, Biotechnology Center, 425 Henry Mall, Madison, WI 53706 **Email:** biogarage@biotech.wisc.edu **Access model:** Access to instruments and workspace in the BioGarage requires a yearly membership. The facility also offers supplementary support services to researchers. For membership options and costs, visit the BioGarage Memberships page on biotech.wisc.edu (specific pricing is not listed publicly — contact biogarage@biotech.wisc.edu for current rates). **Purpose:** Provides affordable access to laboratory space for qualified researchers. Its location within the UWBC building makes it suitable for investigators both within and outside the university community. --- ## Source: https://biotech.wisc.edu/room-reservations/ ### Room Reservations To reserve a room, contact **reservations@biotech.wisc.edu** to inquire about availability. Pricing depends on whether the reservation is for internal or external use. Indicate the purpose of your rental in your inquiry. **Available spaces:** **Auditorium (Room 1111)** - Capacity: up to 135 people - Includes: HD projector, one microphone, A/V support setup, recording capabilities, hybrid talk capabilities - Restrictions: Not allowed for educational classes. No food or drink permitted. - See the A/V Guidelines for details. **Atrium** - Open area for receptions after events - Food allowed - Vendors can set up **Conference Room 1360** - Capacity: ~45 people - Includes: projector, A/V support setup, extension cords - Food and drink permitted - Restrictions: Not allowed for educational classes - See the A/V Guidelines for details. **Note:** The page does not specify hours of operation, advance reservation requirements, or restrictions on who may reserve. Contact reservations@biotech.wisc.edu with specifics. --- ## Cross-facility quick reference | Facility | Director / Lead | Email | Room | |---|---|---|---| | Bioinformatics Resources Core (BRC) | (not listed on public page) | brc@biotech.wisc.edu | — | | Gene Expression Center (GEC) | Sandra Splinter BonDurant | gecinfo@biotech.wisc.edu | 2120 | | DNA Sequencing (NGS) | Joshua R. Hyman | nextgen-seq@biotech.wisc.edu | 1320 | | Mass Spectrometry | Greg Barrett-Wilt | mass_spec@biotech.wisc.edu | 2250 | | Advanced Genome Editing (AGE) | C. Dustin Rubinstein | genomeediting@biotech.wisc.edu | 3122 | | Animal Models (GEAM) | Kathy Krentz | animalmodels@biotech.wisc.edu | 3124 (office), 3210/3220 (labs) | | Isotope Ratio Mass Spec (IRMS / Schoeller Lab) | Michele N. Ravelli | (no public email — use feedback@biotech.wisc.edu) | 2210 | | BioGarage (shared wet lab) | Molly Nellen (Manager) | biogarage@biotech.wisc.edu | 2302 | | Room Reservations | (admin) | reservations@biotech.wisc.edu | — | | General | — | feedback@biotech.wisc.edu | 425 Henry Mall | **Main switchboard:** 608-262-8606 **Common address (all cores):** 425 Henry Mall, Madison, WI 53706 --- ## What this KB does NOT contain The assistant should refuse to answer (and say "I don't know") if asked about: - Specific pricing for sequencing or other core services (only BRC consultation rates are public above) - Turnaround times for any core - Sample volume requirements or input quantity requirements - Detailed sample submission forms or step-by-step submission procedures - Hours of operation for any facility - Parking and directions to 425 Henry Mall - Personnel beyond the directors listed above - Job openings - Pricing for room reservations - Any clinical or diagnostic services (the cores explicitly do NOT provide these) - Anything related to the Biotechnology Training Program (btp.wisc.edu) or MS in Biotechnology Program (ms-biotech.wisc.edu) — those are separate For any of those questions, the assistant should direct users to the appropriate facility email above or to feedback@biotech.wisc.edu.